Short Answer 
To administer a required dose of 100.0 μg (or 0.1 mg) of mRNA, it is essential to know the vaccine concentration, which is 0.20 mg/mL. Using the formula for volume, the needed amount of vaccine suspension is calculated to be 0.5 mL.
Step 1: Understand the Required Dose
First, it’s essential to identify the required dose of mRNA that needs to be delivered. In this scenario, the required dose is 100.0 Œºg (micrograms). To facilitate calculations, it’s useful to convert this to milligrams because the concentration of the vaccine is given in mg/mL.
- 100.0 μg is equal to 100.0 μg / 1000 = 0.1 mg.
Step 2: Know the Vaccine Suspension Concentration
The next step is to recognize the concentration of mRNA in the vaccine suspension. The given concentration is 0.20 mg/mL. This information is crucial for calculating how much volume is needed to obtain the required dose of 0.1 mg of mRNA.
- Concentration of mRNA = 0.20 mg/mL.
- Required Dose = 0.1 mg.
Step 3: Calculate the Volume of Vaccine Suspension
Finally, use the formula to calculate the volume of vaccine suspension needed to deliver the required dose. This calculation is based on the required dose and the concentration of the mRNA in the vaccine suspension.
- Volume of vaccine suspension = Required dose of mRNA / Concentration of mRNA.
- Volume = 0.1 mg / 0.20 mg/mL = 0.5 mL.
Therefore, to administer 100.0 μg of mRNA, 0.5 mL of vaccine suspension should be injected.